Peptide reconstitution calculator
Enter the vial size, the water and your target dose — get the concentration and the exact marks to draw on an insulin syringe. Instant, free, research use only.
Reconstitution calculator
Research calcEnter the vial amount, bacteriostatic water, and target research dose to see concentration and insulin-syringe draw.
| Syringe | Per unit | Units to draw |
|---|---|---|
| U-100 (1 mL = 100u) | 25 mcg | 10 units |
| U-50 (1 mL = 50u) | 50 mcg | 5 units |
| U-40 (1 mL = 40u) | 62.5 mcg | 4 units |
For laboratory research calculation only. Not medical or dosing advice, and not for human consumption.
How reconstitution works
Peptides ship freeze-dried (lyophilised) as a dry powder. Reconstitution means dissolving that powder in bacteriostatic water to make a liquid of known concentration. Two numbers decide everything: how much peptide is in the vial (mg) and how much water you add (mL). Concentration = mg ÷ mL.
New to this? Read our guide on how to reconstitute peptides step by step and storage & stability. Planning a dose by bodyweight? Use the dosage calculator.
Frequently asked questions
How do I use the peptide reconstitution calculator?+
Enter three values: the amount of peptide in the vial (mg), the volume of bacteriostatic water you add (mL), and your target dose (mcg). The calculator returns the concentration (mg/mL and mcg/mL) and the exact number of marks to draw on a U-100, U-50 or U-40 insulin syringe.
What water do you use to reconstitute peptides?+
Research protocols use bacteriostatic water — water with 0.9% benzyl alcohol — because it suppresses bacterial growth and allows a vial to be accessed multiple times. Sterile or distilled water also dissolves the peptide but carries no preservative.
How much water should I add to a 5 mg vial?+
That is your choice and sets the concentration. 2 mL of water in a 5 mg vial gives 2.5 mg/mL (2500 mcg/mL); 1 mL gives 5 mg/mL. More water means a lower concentration and more marks per dose, which makes small doses easier to measure accurately.
Is this calculator medical advice?+
No. It is a calculation aid for research purposes. Peptyds products are supplied strictly for laboratory research and are not for human consumption.